Informe 2 Completo

UNIVERSIDAD CATOLICA LOS ANGELES DE CHIMBOTE FARMACIA Y BIOQUIMICA

GUIA DE PRACTICA DE FARMACOGNOSIA Y FITOQUIMICA MG. Q.F. JAHAIRA DEL ROSARIO BAZALAR

UNIVERSIDAD CATOLICA LOS ANGELES DE CHIMBOTE FACULTAD CIENCIAS DE LA SALUD ESCUELA PROFESIONAL FARMACIA Y BIOQUIMICA

INFORME DE LABORATORIO N°2 ASIGNATURA: FARMACOGNOSIA Y FITOQUIMICA DOCENTE: MG. Q.F. JAHAIRA DEL ROSARIO BAZALAR ESTUDIANTES: AGUIRRE OLIVEROS STIVEN BURGOS SANCHEZ JENNIFFER CIRIACO FLORES JOICE CIER CASTILLO VIOLETA DIAZ VASQUEZ KARINA MALVACEDA SANCHEZ LUIS PEREZ GIL KIMBERLY RODRIGUEZ VASQUEZ ROSARIO ROSARIO LECCA CRISTHIAN SALDAÑA PEÑA SALOME OTINIANO LIÑAN TATIANA VALDIVIEZO ROQUE ALTONELLA

DETERMINACION DE CENIZAS$ HUMEDAD Y TAMIZAJE FITOQUIMICO FITOQUIMICO DE UNA PLANTA MEDICINA V CICLO – SEMESTRE 2!"#!

MAYO 2!"



1. OB OBJE JETI TIVO VO GE GENE NERA RAL: L: • • • •

Determ!"r e# $%!te!&% &e '(me&"& &e (!" &r%)" "# e*t"&% +re*$% , *e$% E*t"-#e$er e# $%!te!&% &e $e!"* &e (!" &r%)" Determ!"r #" $%m/%*$0! (2m$" &e (!" m"ter"# 3e)et"# C%rre#"$%!"r #%* /"r4metr%* +2*$% 5 (2m$% $%! #" $"#&"& &e #" &r%)".

6. DETERM DETERMINA INACIO CION N DE CENIZ CENIZAS: AS: Se e!te!&e /%r $e!"* e# re*&(% (e (e&" &e*/(7* &e #" )!$0! &e (!" &r%)". S( m/%rt"!$" r"&$" e! (e /%r e# (*% !"&e$("&% &e !*e$t$&"*8 #" /%#($0! &e# me&% "m-e!te /%r #%* $%!t"m!"!te*8 (e $%!te!e! *(*t"!$"* &+2$#me!te -%&e)r"&"-#e*8 $%m% *%! "#)(!%* met"#e* /e*"&%*9 , t"m-7! #" /re* /re*e! e!$ $"" &e "&(# "&(#te ter" r"!t !te* e* $%m% $%m% ter terr" r" , "r$ "r$####"8 "8 t"!t t"!t%% e! #"* #"* /#"! /#"!t" t"** me&$!"#e* % "#me!t$"* '"$e! (e *e m"!+e*te (! /%r$e!t"e e#e3"&% &e $e!"* re*/e$t% &e #" m(e*tr" &e re+ere!$". • • • •

OBJETIVOS: C"/"$t"r e! #" t7$!$" &e &eterm!"$0! &e $e!"*. Determ!"r #%* /%r$e!t"e* &e $e!"* &e #"* &r%)"* 3e)et"#e*. Re#"$%!"r e# )r"&% &e $%!t"m!"$0! $%! e# /%r$e!t"e &e $e!"*.

6.6. FUNDAMENTO: L"* $e!"* $e!"* *%! e# re*&(% !%r)4!$% (e (e (e&" &e*/(7* &e*/(7* &e #" &e*tr($$0! &e #" m"ter" %r)4!$"8 (e&"!&% #%* $"t%!e* $%m% $"r-%!"t%*8 *(#+"t%*8 +%*+"t%* re*/e$t3"me!te. 6.;. CENIZAS TOTALES: Permte &eterm!"r #" $"!t&"& &e m"ter"# rem"!e!te &e*/(7* &e #" )!$0!: <$e!"* +*%#0)$"*=8 &er3"&%* &e #%* te&%* &e #"* /#"!t"* , <$e!"* !% +*%#0)$"*= (e *%! e# re*&(% (e (e&" &e*/(7* &e #" )!$0! &e #" m"ter" e>tr"?" @/%#3%8 "re!"8 terr"8 et$. "&'er&%* " #" *(/er+$e &e #" &r%)".



6.;.1. PARTE PARTE EPERIMENTAL. EPERIMENTAL. MATERIALES Y REACTIVOS:       

%&'( '&)*+,A*,--* '&)*+,A/01 '3&+*11 T04-3 ' '5316G(1+**13 C1730*1 ' 7-(,'*151 P1+,-

   



P17'* ' 8+*&(E379&0*1 7'0';1 E<40R'1,&+=-3 71(1 T1<+>1?' 8+&-0)<+,,*-(-8-(<-

PROCEDIMIENTO: De #" m(e*tr" &e #"-%r"t%r%8 $%! e# )r"&% &e trt(r"$0! (e &eterm!" #" !%rm" e*/e$2+$"8 /e*"r 6) $%! &e*3"$0! /erm*-#e &e .m) , *e tr"!*+ere! " (!" $4/*(#" &e /%r$e#"!" /re3"me!te t"r"&" , *e$"&"8 *e $"#e!t" , *e &e*e$" " 1C &(r"!te 6 '%r"*. L" $4/*(#" *e $%#%$" e! (!" &e*e$"&%r" &%!&e *e e!+r2" " tem/er"t(r" "m-e!t"# , *e /e*"8 $%#%$4!&%*e !(e3"me!te e! #" e*t(+" &(r"!te 1 '%r"8 3%#37!&%*e " /e*"r '"*t" %-te!er (!" m"*" $%!*t"!te. 6.. TAMIZAJE FITOQUIMICO: FUNDAMENTO: Se +(!&"me!t" e! #" e>tr"$$0! *e#e$t3" &e #%* /r!$/%* "$t3%* $%!te!&%* e! (! m"ter"# 3e)et"#8 "/r%3e$'"!&% *( *%#(-#&"& e! &+ere!te* *%#3e!te*8 em/e"!&% #" e>tr"$$0! e! *%#3e!te* &e me!%r /%#"r&"& $%m% e# 7ter et2#$%8 (! *%#3e!te &e me&"!" /%#"r&"& $%m% e* e# "#$%'%# et2#$% , +!"#me!te $%! (! *%#3e!te &e "#t" /%#"r&"& $%m% e* e# ")(". C"&" (!% &e & e #%* e>tr"$t%* ",(&"&%* /%r #" m$r%(2m$" /ermte &e!t+$"r#%* me&"!te #" +%rm"$0! &e /re$/t"&%*8 $%#%r"$0!8 et$. E*t"* re"$$%!e* *e $"r"$ter"! /%r *er *e#e$t3"* /"r" #"* $#"*e* % )r(/%* &e $%m/(e*t%* (e *e !3e*t)"!8 *%! *m/#e* , r4/&"*8 &ete$t"! #" m2!m" $"!t&"& /%*-#e , (t#"! (! m2!m% &e e(/% &e #"-%r"t%r%.



6..1. OBJETIVOS:

         

Determ!"r me&"!te e# t"m"e +t%(2m$% #" /re*e!$" &e met"-%#t%* *e$(!&"r%* Ver+$"r #" /re*e!$" &e *(*t"!$"* $%!%$&"* e! e*/e$e* me&$!"#e* $%!%$&"*. 6..6. MATERIALES Y REACTIVOS: ter et2#$% A#$%'%# et2#$% A)(" &e*t#"&" T(-%* &e e!*",% Gr"&##"* P"/e# &e +#tr% E*/4t(#" /e(e?" Em-(&% Re"$t3%* /"r" T"m"e +t%(2m

%$6..;. PROCEDIMIENTO:

6) MP Et6O /"

%$ETRACTO

RESIDUO EtOH

ETEREO

ETRACTO ETANOLICO

RESIDUO  H6O



ETRACTO ACUOSO

6... ETRACTO ETEREO: • • •

E!*",% &e Dr")e!&%r++ , M",er "r" "#$"#%&e* E!*",% &e B"#et8 /"r" #"$t%!"* ,% $(m"r!"* E!*",% &e Le-erm"!B($'"r&8 /"r" trter/e!%* ,% e*tere%&e*

6... ETRACTO ETANOLICO: • • • •

E!*",% &e Fe'#!) /"r" "K$"re* re&($t%re* E!*",% &e C#;Fe /"r" +e!%#e* , t"!!%* E!*",% &e S'!%&" /"r" +#"3%!%&e* E!*",% &e Dr")e!&%r++ , M",er /"r" "#$"#%&e*

6... ETRACTO ACUOSO: • • • •

E!*",% &e Dr")e!&%r++ , M",er /"r" "#$"#%&e* E!*",% &e C#;Fe /"r" +e!%#e* , t"!!%* E!*",% &e S'!%&" /"r" +#"3%!%&e* E!*",% &e Fe'#!) /"r" "K$"re* re&($t%re*

6.. RESULTADOS: 

Determ!"r e# /%r$e!t"e &e '(me&"& &e #"* &r%)"* 3e)et"#e*

Se /e*0 6)r &e m(e*tr"
RESIDUO



A)re)"m%* et"!%# '"*t" e# r"* &e# m"tr"

E# +#tr"&% &e #" m(e*tr" *e '"$e /%r ; 3e$e*





Determ!"r me&"!te e# t"m"e +t%(2m$% #" /re*e!$" &e met"-%#t%* *e$(!&"r%*

E@TRACTO ETEREO: D(1/'5-(8& 71(1 1*,1*-+'3: E! (! t(-% &e e!*",% *e 3erte 6 m# &e MP + 6 " ; )t* &e &r")e!&%r+t. D% $%m% re*(#t"&%  /%*t3%* , &e $%#%r "!"r"!"&% &em%*tr"!&% #" /re*e!$" &e "#$"#%&e*.

O V I T I S O P



M16'(: E! (! t(-% t(-% &e e!*", e!*",%% *e 3ert 3ertee 6 m# &e MP + 6 " ; )t* &e M",er. D% $%m% re*(#t"&% 6 /%*t3%* , &e $%#%r -#"!$% #e$'%*% &em%*tr"!&% #" /re*e!$" &e "#$"#%&e*.

O V I T I S O P

E5316- ' B1*?'&$ 71(1 *1,&-513 6- ,0<1(+513: E! (! t(-% &e e!*",% *e 3erte 6 m# &e MP + . m# &e "$&% /r$% + . m# &e N" OH. D% $%m% re*(#t"&% #" re"$$0! /%*t3" , &e $%#%r !"r"!" &em%*tr"!&% #" /re*e!$" &e #"$t%!"* , $(m"r!"*.

O V I T I S O P

E5316- ' L+'4'(<15#B0,1($ 71(1 &(+&'(7'5-3 6- '3&'('-+'3: E! (! t(-% &e e!*",% *e 3erte 6 m# &e MP (e>tr"$t% etere%)+ . m# &e $#%r%+%rm% + . m# &e "!'2&r&% "$7t$% , /%r #"* /"re&e*  "  )t* &e "$&% *(#+Kr$% , !% *e O V I T A G E N



")t". D% $%m% re*(#t"&% #" !% +%rm"$0! &e# "!##% 3%#4$e% &em%*tr"!&% "* #" !% /re*e!$" &e trter/e!%* , ⁄ % e*ter%&e*.

E@TRACTO ETANOLICO: E5316- ' F'*+5/ 71(1 1>,1('3 ('0,&-('3 E! e# /rmer t(-% &e e!*",% *e 3erte 6m# &e #" MP + . m# &e +e'#!) A , . m# &e +e'#!) +e'#!) B8 /%*ter% /%*ter%rr " e*% *e #e ##e3" ##e3" " -"?% -"?% m"r". m"r". D% $%m% $%m% re*(#t"& re*(#t"&%% (!" re"$ re"$$ $0! 0! !e)" !e)"t t3" 3" &%!& &%!&ee &em( &em(e* e*tr tr"" (e (e !% '", '", #" /re*e re*e!$ !$" " &e "($ "($"r "re* e* re&($t%re*.

O V I T A G E N

E5316- ' C*F' 71(1 8'5-*'3 6 &15+5-3



E! (! t(-% &e e!*",% *e 3erte 6 m# &e #" MP + 6 " ; )t* &e C# ;Fe. D% $%m% re*(#t"&% 6 /%*t3% , &e $%#%r 3er&e &em%*tr"!&% "* #" /re*e!$" &e +e!%#e* , t"!!%*.

O V I T I S O P

E5316- ' S+5-1 71(1 8*1=-5-+'3 E! (!" $"/*(#" &e /%r$e#"!" *e "?"&e 6m# &e #" MP + #m"&(r" &e M) + .m# &e "$&% $#%r'2&r$%. D% $%m% re*(#t"&% 1 /%*t3% &e $%#%r r%%8 &em%*tr4!&%*e "* #" /re*e!$" &e +#"3%!%&e*.

O V I T I S O P

E5316- ' D(1/'5-(88 6 M16'( 71(1 1*,1*-+'3 D(1/'5-(8&: E! (! t(t(-% &e e!*" e!*",,% *e 3e 3erte rte 6 m# &e MP + 6 " ; )t* &e &r")e!&%r+t &r")e!&%r+t.. D% $%m% re*(#t"&% re*(#t"&% 1 /%*t3% /%*t3% , &e $%#%r "!"r"!"& "!"r"!"&%% &em%*tr"!& &em%*tr"!&%% #" /re*e!$" &e "#$"#%&e*.

O V I T I S O P

E@TRACTO ACUOSO:



E5316- ' D(1/'5-(88 6 M16'( 71(1 1*,1*-+'3 D(1/'5-(8&: E! D(1/'5-(8&: E! (! t(-% &e e!*",% *e 3erte 6 m# &e MP + 6 " ; )t* &e &r")e!&%r+t. D% $%m% re*(#t"&% 1 /%*t3% , &e $%#%r "!"r"!"&% &em%*tr"!&% #" /re*e!$" &e "#$"#%&e*.

O V I T I S O P

E5316- ' C*F' 71(1 8'5-*'3 6 &15+5-3 E! (! t(-% &e e!*",% *e 3erte 6 m# &e #" MP + 6 " ; )t* &e C# ;Fe. D% $%m% re*(#t"&% #" re"$$0! !e)"t3" !e)"t3" &em%*tr"!&% "* #" !% /re*e!$" &e +e!%#e* , t"!!%*.

O V I T A G E N

E5316- ' S+5-1 71(1 8*1=-5-+'3



E! (!" $"/*(#" &e /%r$e#"!" *e "?"&e 6m# &e #" MP + #m"&(r" &e M) + .m# &e "$&% $#%r'2&r$%. D% $%m% re*(#t"&% #" re"$$0! !e)"t3"8 &em%*tr4!&%*e "* #" !% /re*e!$" &e +#"3%!%&e*.

O V I T A G E N

6.. DISCUSION: •

•

E# t"m"e +t%(2m$% *e re"#0 $%! e# %-et3% &e &eterm!"r #" /re*e!$" &e &eterm!"&%* met"-%#t%* *e$(!&"r%*8 e! &e/e!&e!$" &e *(* $"r"$ter2*t$"* e*tr($t(r"#e* , *%#(-#&"& &e $"&" (!% &e e##%*8 (e /ermt"! *( &e!t+$"$0! e! (!% ( %tr% *%#3e!te @")("8 "#$%'%# , 7ter. E! #" t"-#" 1 *e m(e*tr"! #%* re*(#t"&%* &e# t"m"e +t%(2m$% re"#"&% " #%* e>tr"$t%* et7re%8 et"!%#$% , "$(%*% &%!&e *e m(e*tr" #" "#t" 3"r"-#&"& &e $%m/(e*t%* /re*e!te* e! #" /#"!t" &e# /"$%9 &e*t"$4!&%*e e!tre e*t%*: /(m"r!"* , #"$t%!"*8 "#$"#%&e*8 +e!%#e* +e!%#e* , t"!!%*8 "K$"re* re&($t%re*.


M'&1 M' &144-*+*+&&-33

E&( E &(1, 1,&&- E& E&'( '(''-

E5316- ' D(1/'5-(8&

R'1,,+-5

E5316- ' M16'(

R'1,,+-5

E5316- ' B1*?'&

R'1,,+-5

E5316- ' L+'4'(<15# B0,1(

R'1,,+-5


E&(1, E&( 1,&&E&15-*+,-

F'*+5/

R'1,,+-5

E5316- ' C*F'

R'1,,+-5

E5316- ' S+5-1

R'1,,+-5

D(1/'5-(8&

R'1,,+-5

M16'(

R'1,,+-5

E&(1,&- A,0-3-

D(1/'5-(8&

R'1,,+-5

M16'(

R'1,,+-5

E5316- ' C*F'

R'1,,+-5

S+5-1

R'1,,+-5









L%* e*/"$%* e! -#"!$% *)!+$"! (e e*%* e!*",%* !% *e #e re"#"r%! "# e>tr"$t%. E# *)!%  *)!+$" (e *e %-t(3% (!" re*/(e*t" /%*t3" /"r" e*e met"-%#t% e! e# e>tr"$t%. E# *)!%  *)!+$" (e *e %-t(3% (!" re*/(e*t" !e)"t3" /"r" e*e met"-%#t% e! e# e>tr"$t%. Se /(e&e re*(mr (e *e %-t(3er%! re*(#t"&%* /%*t3%* /"r" (!" (!" )r"! &3er*&"& &e $%m/(e*t%*8 #% (e /(&er" e>/#$"r #" "#t" (t#&"& "tr-(&" "# /"$% /"r" #" $(r" &e &+ere!te* "+e$$%!e*. "+e$$%!e*.



6.. CONCLUSIONES: • • •

Se &eterm!0 e# $%!te!&% &e '(me&"& &e (!" &r%)" "# e*t"&% +re*$% , *e$%. Se &eterm!% #" $%m/%*$0! (2m$" &e (!" m"ter"# 3e)et"#. Se &eterm!% me&"!te e# t"m"e +t%(2m$% #" /re*e!$" &e met"-%#t%* *e$(!&"r%*

6.. CUESTIONARIO: E30'<1&+,' -&(1 <1(,1 8+&-0)<+,1$ ='5&1?13 6 '3='5&1?13 ,-5 ('37',&- 1 *1 '8',&011 '5 7(9,&+,1



Ve!t""* , De*3e!t""*

L" M"#3" *#3e*tr* /%*ee )r"!&e* /r%/e&"&e* $(r"t3"* t"#e* $%m%: em%#e!te8 "!t$"t"rr"#8 "!t!+#"m"t%r% , &)e*t3"8 )r"$"* " *(* *(*t"!$"* +t%(m$"* $%m% #%* "#$"#%&e*8 #"$t%!"8 trter/e!%*8 e*ter%#e*8 "!t%$"!!"* , *"/%!!"*. N% *%#% /"r" $"#m"r &%#e!$"* * !% /"r" *( (*% &%me*t$%. 

T%>$&"&.

A(!(e !% '" *&% re/%rt"&" $%m% t%>$" e! '(m"!%*8 e>*te! eem/#%* &e e!3e!e!"me!t%* *er%* , +"t"#e* e! "!m"#e* (e #" '"! $%m&% (!t% $%! e# /"*t%.



Q0 +5+,1 *1 7('3'5,+1 '*'=11 ' ,'5+>13 '5 051 (-/1 ='/'&1* I!&$" )e!er"#me!te (! /r%$e&me!t% &e re$%#e$$0! , "#m"$e!"me!t% !"&e"$("&%. O t"m-7! /(e&e '"-er #" /re*e!$" &e "re!" % terr" e! #" &r%)" 3e)et"#. Q0 +<7-(&15,+1 &+'5' *1 '&'(<+51,+5 ' *1 ,-<7-3+,+5 0)<+,1 ' 051 (-/1 ='/'&1* E* (! "*/e$t% +(!&"me!t"#8 e# e*t(&% &e #" $%m/%*$0! (2m$" *e $%!%$e $%m% Ft%(2m$". Se '"$e e!*",%* $("#t"t3%* /"r" ##e)"r " #%* /r!$/%* "$t3%* (e *e e!$(e!tr"! e! (!" &r%)". T"m-7! *e '"$e e!*",%* /"r" $"r"$ter"r $"r"$ter"r , 3"#%r"r #%* /r!$/%* "$t3%*. Q0 +<7-(&15,+1 &+'5' *1 '&'(<+51,+5 ' 0<'1 '5 051 (-/1 ='/'&1* E# e>$e*% &e ")(" e! &r%)"* 3e)et"#e* e* e# re*/%!*"-#e &e# $re$me!t% &e -"$ter"* , '%!)%*8 , t"m-7! &e #" '&r0#** &e *(* $%!*tt(,e!te*. E# e>$e*% &e ")(" $"(*" *( &eter%r%. E# $%!te!&% &e ")(" *e &eterm!" /%r e# m7t%&% )r"3m7tr$%8 /r%$e*% me&"!te e# $("# #" &r%)" *e *e$" " 1C '"*t" (! /e*% $%!*t"!te8 /%r #% (e *e $%!*&er" (! m7t%&% &e*tr($t3% &e# & e# m"ter"# 3e)et"# "!"#"&%.

Q0 -&(-3 '5316-3 8)3+,-3 3' 70''5 1,'( 1 051 (-/1 ='/'&1* E!*",%* +2*$%* %r)"!%#7/t$%* , m"$r%*$0/$%* &%!&e *e /(e&"! e3&e!$"r #"* /"re&e* &e $e#(#%*".



6.1. BIBLIOGRAFIA: • •

Tre"*e. F"rm"$%)!%*". F"rm"$%)!%*". De$m" e&$0! @Prmer" e! E*/"?%#. E*/"?%#. C%m/"?2" E&t%r"# $%!t!e!t"# S. A.8M7>$%. 1 "##*8 T.E. M"!("# &e F"rm"$%)!%*". Tr"&($$0! &e #" $("rt" e&$0! e! !)#7*. C%m/"?2" E&t%r"# C%!t!e!t"# S. A.8 M7>$%.1

Informe 2 Completo

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